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Then the cells were permeabilized with blocking/permeabilizing solution (50 mM NH 4 Cl, 0.1% Saponin, 1% BSA in PBS), and an Alexa568-conjugated secondary antibody was used to label endocytosed anti-PrP antibody bound to its ligand

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A landmark 2007 study published in the Journal of Investigative Dermatology found that AHK-Cu at concentrations between 10⁻¹² and 10⁻⁹ M stimulated dermal papilla cell (DPC) proliferation, elongated human hair follicles ex vivo, and shifted the Bcl-2/Bax ratio in favor of cell survival — meaning fewer follicle cells died prematurely [2]